Analysis Note

ControlCells expressing the His-tag

Application

This Anti-Histidine Tagged Antibody (anti His antibody) clone 4D11 is validated for use in IC, WB for the detection of Histidine Tagged.

Immunogen

N-terminal His-tagged and truncated TRAP protein corresponding to amino acids 26-248 from P. falciparum expressed in E coli.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, and 0.07% sodium azide.

Format: Purified

Quality

routinely evaluated by immunoblot on recombinant N-terminal and C-terminal His-tagged proteins

Specificity

Recognizes tag sequence HHHHHHGS on either the N- or C-terminus of recombinant proteins; GS amino acids are required for recognition of 6His tag

Target description

The apparent molecular weight is related to the tagged protein.